Long PCR: Selective Suppression by Restriction Endonuclease Digestion
نویسندگان
چکیده
منابع مشابه
Long PCR: selective suppression by restriction endonuclease digestion.
The polymerase chain reaction (PCR) has become the most widely used technique for the manipulation of DNA with applications for cDNA cloning, gene cloning, polymorphism detection, mutagenesis and allele-specific diagnosis (8). All of those applications have been extended significantly by the recent development of “long” PCR, a technique that makes it possible to amplify DNA fragments up to 40 k...
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The polymerase chain reaction (PCR) is the most widely used technique for the study of DNA. Applications for PCR have been extended significantly by the development of "long" PCR, a technique that makes it possible to amplify DNA fragments up to 40 kb in length. This article describes two novel applications of the long PCR technique, one which simplifies restriction mapping and another which en...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96215bm01